Coding

Part:BBa_K1179007:Design

Designed by: Nelson Hall   Group: iGEM13_MIT   (2013-09-15)


(Acyl-TyA-TCS-rtTA3-His) A membrane/exosome targeting rtTA3 fusion with a TEV clevage site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 704
    Illegal BamHI site found at 1351
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 604
    Illegal PstI site found at 853
    Illegal PstI site found at 875
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Keep in mind that the Acyl-TyA sequence exports the protein into exosomes so protein extraction and other quantification protocols are needed to test successful experimental function.

Source

The TyA protein is derived from a yeast retrotransposon virus-like particle and when added with the Acylation tag it acts as an exosome export protein, allowing the rtTA to be exported when fused to the TyA. Also was synthesized with a TEV cleavage site in between the TyA and rtTA sequences.

References